Part:BBa_K902068

RhaS

RhaS is a transcription factor which is part of the rhaSR operon.

Usage and Biology

RhaS activates expression of the rhaBAD operon on the P_rha rhamnose promoter. In order for it to bind to P_rha, the CRP-cAMP (cAMP receptor protein) complex must be bound to the promoter. Additionally, rhamnose will improve function of RhaS (Egan & Schlief, 1993).

In its native E. coli, the rhaS (on the rhaSR operon off the pRha promoter) is upregulated by the RhaR-rhamnose complex (Egan & Schlief, 1993).

The [http://2012.igem.org/Team:Calgary iGEM Calgary 2012 team] intended to upregulate rhaS so that they could use the rhamnose promoter to activate a kill switch without rhamnose necessarily being present in the environment. Please view our [http://2012.igem.org/Team:Calgary/Project/HumanPractices/Killswitch/Regulation#rhamnose Wiki] for more design details of the kill switch.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 440
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 172
  
• 1000
  COMPATIBLE WITH RFC[1000]