Part:BBa_K844017

Spider Silk Generator - 10x "F" Silk Construct with His Tag

Protein generator part for Spider Silk. Protein is a 10x fusion of spider silk "F" subunits (1x BBa_K844007 followed by 9x BBa_K844003), which were codon optimized to use a minimal set of tRNA codons (1 codon per amino acid). Contains a lactose/IPTG inducible promoter, RBS, and a 10x-His tag (BBa_K844000) for purification of protein.

IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below and on composite parts using this part will not reflect this scar sequence (it shows Assembly Standard #10 scars).

Related Parts

BBa_K844000 - A 10x-His tag with double stop codons (TAATAA) which was vital to the creation of the gel and blot shown above.

BBa_K844002 - The non-optimized native spider silk subunit that K8440016 is a codon optimized and improved concatemer of.

BBa_K844016 - Another silk generator, this part uses the "B" subunit and has only 4x repeat units.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]