Part:BBa_K934012

Plas-LuxI

We constructed this part by combining BBa_K649000 and BBa_K081008. This part generates LuxI enzyme in the presence of LasR-3OC12HSL complex.

This work was done by M.M, X.T and S.L.

To characterize Plas-LuxI (BBa_K934012), we introduced Plas-LuxI on pSB3K3 with Ptrc-LasR on pSB6A1 to E.coli as “3OC12HSL dependent 3OC6HSL producer cell”. In this E.coli, constitutively expressed LasR activates the expression of LuxI in the presence of 3OC12HSL. We then introduced Ptet-LuxR (BBa_S03119) and Plux-GFP (BBa_K395100) to E.coli as a “Lux reporter cell”.

When the supernatants of the culture of “3OC12HSL dependent 3OC6HSL producer cell” were induced to “Lux reporter cell”, GFP expression in “Lux reporter cell” was activated. This result shows that Plas-LuxI (BBa_K934012) is fuctioning.

We improved a previous part Plas-LuxI (BBa_K266000)

and accomplished a positive feedback system with our new part Plux-LasI (BBa_K934022).

This work was done by M.M, X.T and S.L.

For more information, see [http://2012.igem.org/Team:Tokyo_Tech/Project our work in Tokyo_Tech 2012 wiki].

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 749
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Unknown
• 25
  INCOMPATIBLE WITH RFC[25]

  Unknown
• 1000
  COMPATIBLE WITH RFC[1000]