Reporter

Part:BBa_K395100

Designed by: Shohei Kitano   Group: iGEM10_Tokyo_Tech   (2010-10-05)

GFP reporter activated by LuxR and 3OC6HSL (R0062:K121013)

This part is GFP reporter which is activated by LuxR and 3OC6HSL, and the promoter is R0062. And we use this part to measure the activity of BBa_R0062.

Tokyotech LuxR activation promoter assay(R0062).jpg
Tokyotech LuxR ractivatio promoter assay2.jpg

In order to measure the activity of R0062, we introduced S03119 (pTetR-rbs-luxR-ter) on pSB3K3 and K395100 into DH5α. We confirmed that R0062 is activated by 3OC6HSL and LuxR as we expected.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/lux_act_rep more information])











Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 89
    Illegal BamHI site found at 78
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 781


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Categories
Parameters
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