DNA
pSC101

Part:BBa_I50040:Design

Designed by: Reshma Shetty   Group:   (2006-02-03)
Revision as of 21:56, 9 June 2006 by Rshetty (Talk | contribs) (→‎Design Notes)


nonfunctional pSC101 origin of replication


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Two mutations were introduced in order to remove two MfeI sites. These two mutations occur within an inverted repeat and one also occurs within a ribosome binding site. It is unclear what impact these mutations will have on the replication origin.

Source

References

  • R. Lutz and H. Bujard. Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I-I regulatory elements. Nucleic Acids Res, 25:1203,10, 1997.