Generator
PatgF1GFP

Part:BBa_K844015

Designed by: Brian Smith   Group: iGEM12_Utah_State   (2012-10-02)
Revision as of 04:32, 3 October 2012 by Canyoncatalyst (Talk | contribs)

lac/IPTG inducible Spider Silk 1x "F" Subunit fused to GFP

This part is a spider silk 1x "F" subunit (BBa_K844007) with GFP (BBa_K208000) fused to its C-terminus. This generator is lactose/ITPG inducible.

IMPORTANT NOTE: This part uses Assembly Standard #23 (Silver Fusion) scar sites, which the sequence data below will not reflect (it shows Assembly Standard #10 scars).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 443
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 962
    Illegal XhoI site found at 863
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//collections/silk
Parameters
None