Composite
Part:BBa_K781000
Designed by: Kevin Chen Group: iGEM12_Queens_Canada (2012-10-01)
[R0010][B0034] - E. coli K12 FliC
This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer. Or, this part can be used as a template for PCR overlap extension, which will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1452
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 529
Illegal AgeI site found at 937 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | [R0010][B0034] - E. coli K12 FliC |