Part:BBa_K781000

[R0010][B0034] - E. coli K12 FliC

This is the complete flagellin coding sequence with biobrick standard cut sites removed, under the promoter R0010 and RBS B0034. This part can be used to over express the flagellin monomer.

The main use of this part is for PCR overlap extension, which will make an insertion at the annotated site, given that the insert is in the appropriate format. The resulting chimera will express protein immediately after the PCR reaction is transformed.

This part has been previously characterized as BBa_K777109. The part that we have submitted has some slight differences in the silent mutations made to remove Biobrick cut sites. This part also includes the promoter R0010 and the RBS B0034.

This part was used for in PCR overlap extension[1] to give the following parts: BBa_K781000, BBa_K781002, BBa_K781003, BBa_K781004 and BBa_K781005.

1. Anton V. Bryksin and Ichiro Matsumura. (2010) Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids. BioTechniques, Vol. 48, No. 6, pp. 463–465

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1452
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 529
  Illegal AgeI site found at 937
  
• 1000
  COMPATIBLE WITH RFC[1000]