Coding
SYFP2

Part:BBa_K864100

Designed by: Erik Gullberg   Group: iGEM12_Uppsala_University   (2012-09-24)
Revision as of 18:52, 29 September 2012 by Erigu863 (Talk | contribs)

Super Yellow Fluorescent Protein 2 (SYFP2)

This part codes for the bright yellow fluorescent protein SYFP2. SYFP2 is a GFP based monomeric protein with optimized folding, maturation and a narrow fluorescence emission spectrum with a maximum of 515 nm. Bacteria expressing SYFP2 are reported to be 12 times brighter than those expressing EYFP(Q69K) and almost 2-fold brighter than bacteria expressing Venus (Kremer et al 2006). Mutations compared to wtGFP amino acid sequence (GenBank Accession number M62653) are F46L F64L S65G S72A M153T V163A S175G T203Y A206K. See the article listed in references. The sequence has been codon optimized for expression in E coli by DNA 2.0.

Kremers et al reported the following data:

Excitation peak: 515 nm. Emission peak: 527 nm. Relative brightness to EYFP2(Q96K) in E coli: 12.0. Extinction Coefficient (M-1 cm-1): 101000. Quantum Yield: 0.68.

Usage and Biology

This part is useful as a reporter.

SYFP2 expression.jpg

iGEM12_Uppsala_University. Fluorescence on UV table. The images above show E coli constitutively expressing SYFP BBa_K864100 using promoters with different strengths.

References

[http://pubs.acs.org/doi/abs/10.1021/bi0516273] Kremers, G.-J., Goedhart, J., van Munster, E. B., & Gadella, T. W. J. (2006). Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry, 45(21), 6570–6580. doi:10.1021/bi0516273

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/reporter/yfp
//collections/chromoprotein/uppsala
//function/reporter/fluorescence
Parameters
chassisCodon optimized for expression in E coli.
colorYellow
emission527 nm
excitation515 nm