Coding

Part:BBa_K801090:Design

Designed by: Ingmar Polte   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 22:37, 26 September 2012 by Mary (Talk | contribs) (→‎References)

phenylalanine ammonia lyase (PAL) + yeast consensus sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2096
    Illegal BamHI site found at 358
    Illegal XhoI site found at 409
    Illegal XhoI site found at 472
    Illegal XhoI site found at 490
    Illegal XhoI site found at 568
    Illegal XhoI site found at 769
    Illegal XhoI site found at 1012
    Illegal XhoI site found at 1759
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 928
    Illegal NgoMIV site found at 1174
    Illegal NgoMIV site found at 1342
    Illegal NgoMIV site found at 1485
    Illegal NgoMIV site found at 1819
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 469
    Illegal BsaI site found at 799
    Illegal BsaI site found at 805
    Illegal BsaI.rc site found at 1930
    Illegal SapI site found at 759



Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nuccore/AX366866

PMID: 15932991


Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase


Abbreviations: PAL = Phenylalanine Ammonia Lyase

Design Notes

Related BioBrick:

Quality control measures:
Part was totally sequenced

Backbone:
Backbone name: pSB1C3 Resistance: Cp

Protein coding:
Protein: Phenylalanine Ammonia Lyase

Enzymatic activity: EC-number 4.3.1.25

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:

  • Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA


Organism:

  • Genesequence derived from Rhodosporidium toruloides'

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AX366866 GenBank: Sequence 18 from Patent WO0208402]

[http://www.uniprot.org/uniprot/P11544 Uniprot: Phenylalanine/tyrosine ammonia-lyase]