Part:BBa_K801090:Design

phenylalanine ammonia lyase (PAL) + yeast consensus sequence

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2096
  Illegal BamHI site found at 358
  Illegal XhoI site found at 409
  Illegal XhoI site found at 472
  Illegal XhoI site found at 490
  Illegal XhoI site found at 568
  Illegal XhoI site found at 769
  Illegal XhoI site found at 1012
  Illegal XhoI site found at 1759
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 928
  Illegal NgoMIV site found at 1174
  Illegal NgoMIV site found at 1342
  Illegal NgoMIV site found at 1485
  Illegal NgoMIV site found at 1819
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 469
  Illegal BsaI site found at 799
  Illegal BsaI site found at 805
  Illegal BsaI.rc site found at 1930
  Illegal SapI site found at 759
  

Plasmid (template for PCR) provided by John A. Morgan, School of Chemical Engineering, Purdue University, West Lafayette, Indiana, USA

http://www.ncbi.nlm.nih.gov/nuccore/AX366866

PMID: 15932991

Keywords: Coumaryol-CoA, Coumaroyl-Coenzyme A, Xanthohumol, Biosynthesis, 4-coumarate-coenzyme A ligase

Abbreviations: PAL = Phenylalanine Ammonia Lyase

Design Notes

Related BioBrick:

Other versions: BBa_K801091: PAL coding region

Related BioBricks:

• BBa_K801091: phenylalanine ammonia lyase (PAL) coding region
• BBa_K801092: 4-coumarate - coenzym A ligase (4CL) + yeast consensus sequence
• BBa_K801093: 4-coumarate--coenzyme A ligase (4CL) coding region
• BBa_K801094: Naringenin - chalcone synthase (CHS) + yeast consensus sequence
• BBa_K801095: Naringenin - chalcone synthase (CHS) coding region
• BBa_K801096: Aromatic prenyltransferase (APT) coding region
• BBa_K801097: Chalcone O-methyltransferase (OMT1) + yeast consensus sequence
• BBa_K801098: O-methyltransferase 1 (OMT1) coding region

Cloning details:
Designed in RFC10

Quality control measures:
Part was totally sequenced

Backbone:

• Backbone name: pSB1C3
• Resistance: Cp
• Copynumber: high

Protein coding:

• Protein: Phenylalanine Ammonia Lyase
• Tag: c-terminally fused Strep

Enzymatic activity: EC-number 4.3.1.25

Cytotoxicity:

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:
Plasmid: pKS2µHyg-PAL-4CL-CHS, provided by John A. Morgan, Purdue University, USA

Organism:
Genesequence derived from Rhodosporidium toruloides'

References

Literature references:
[http://www.ncbi.nlm.nih.gov/pubmed/15932991 Pubmed : Jiang, H., Wood, K.V., Morgan, J.A., 2005. Metabolic Engineering of the Phenylpropanoid Pathway in Saccharomyces cerevisiae. Appl. Environ. Microbiol. 71, 2962–2969]

Database references:
[http://www.ncbi.nlm.nih.gov/nuccore/AX366866 GenBank: Sequence 18 from Patent WO0208402]

[http://www.uniprot.org/uniprot/P11544 Uniprot: Phenylalanine/tyrosine ammonia-lyase]