Coding

Part:BBa_K909009

Designed by: Gintautas Vainorius   Group: iGEM12_ETH_Zurich   (2012-09-23)
Revision as of 18:26, 26 September 2012 by Gintas (Talk | contribs)

cDNA of UV-B sensing protein UVR8 from Arabidopsis thaliana


UVR8 is responsible for UV-B detection in plants. In dark state UVR8 forms a dimer, which breaks into monomers upon exposure to UV-B light (280-315 nm). Later monomer induces transcriptional changes necessary for UV-B damage repairs and protection. In addition a BamHI site is inserted after ATG codon for fusions with other proteins (e.g. tetR-DNA binding domain (BBa_K909007)), to use UVR8 as a UV-B sensing domain (BBa_K909008).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 76
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 560
    Illegal PstI site found at 1214
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 91


[edit]
Categories
//cds/receptor
Parameters
None