Part:BBa_K907005

Dual phase protein generator(mRFP default). mRFP and GFP

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<Part Description>
This part is derivative of BBa_K907003, twins with BBa_K907005

BBa_E1010(mRFP, reversed) and BBa_0040(wtGFP) are attached to both ends of BBa_K907003 by overlapping extension PCR. This part normally generates mRFP in E.coli(strain MG1655). When BBa_K907000(Bxb1 integrase) inverts the promoter orientation, it starts to generate GFP.

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<Part Demonstration>

Figure 1. Experimental results of BBa_K907005. Two ep-tubes designated as BBa_K907005 contain centrifuged E.coli MG1655 cells possessing BBa_K907005. When the MG1655-BBa_K907005 is double transformed with BBa_K907000, expressed in pTrcHis2A vector which means under control of Trc promoter, color of cells changed into pink rather than red of MG1655-BBa_K907005, 12hours of incubation in LB after 1mM of IPTG induction.

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<Related Parts>
BBa_K907000 - Mycobacterium Phage Bxb1 integrase
BBa_K907001 - Mycobacterium Phage Bxb1 excisionase
BBa_K907002 - Binary Signal Generator, RBS(reverse) - attB - Promoter - attP - RBS
BBa_K907003 - Binary Signal Generator, Promoter Reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS
BBa_K907004 - Dual Phase Protein Generator(GFP default). mRFP and GFP

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Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 750
  Illegal NheI site found at 773
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 838
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 703
  Illegal AgeI site found at 10
  Illegal AgeI site found at 122
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 723
  Illegal BsaI.rc site found at 806
  Illegal BsaI.rc site found at 1499