Coding
LIMS1

Part:BBa_I742110:Experience

Designed by: sarah hollingshead   Group: iGEM07_Edinburgh   (2007-10-19)
Revision as of 17:42, 26 September 2012 by Simon Heinze (Talk | contribs) (→‎Applications of BBa_I742110)

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Please enter how you used this part and how it worked out.

Applications of BBa_I742110

A composite LIMS1 part with a Lac promoter has been characterized. For details, please visit this page.

Team TU Munich 2012 used this part for expression of limonenesynthase and added a yeast consensus sequence for improved expression as well as a Strep-Tag for simple purification or detection via western blot. See BBa_K801060. Since the DNA of BBa_I742110 is not available and we submitted our BioBrik BBa_K801060 we improved this part by making limonene synthase physically available in the registry.

User Reviews

UNIQd9e025d6583fdf62-partinfo-00000002-QINU

No review score entered. iGEM TU Munich 2012

BBa_K118025 is based on this part. British Columbia statet on the experience page that they could not detect the expression of Limonene-Synthase or the production of limonene. We report that we were not able to express Limonene-Synthase, but also that we showed its functionality both in vitro and in vivo. See our Biobrick BBa_K801060.

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UNIQd9e025d6583fdf62-partinfo-00000005-QINU