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Coding

Part:BBa_K801060:Design

Designed by: Lara Kuntz   Group: iGEM12_TU_Munich   (2012-09-21)
Revision as of 17:22, 26 September 2012 by Mary (Talk | contribs) (→‎Design Notes)

(+)-Limonene synthase 1 with Strep-tag and yeast consensus sequence.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1683
    Illegal SapI.rc site found at 107


Keywords:


Abbreviations:

Design Notes

Related BioBrick:

Quality control measures:

Backbone:

Protein coding:

Enzymatic activity:

Cytotoxicity:
This Biobrick leads to the production of limonene. We investigated the toxicity of limonene and found out, that the growth of yeast strains was inhibited at concentrations of 1 mM. Limonene with a concentration of 100 mM even showed a lethal effect. Lower concentrations of limonene (range of mikro molar) did not show any growth inhibition or effect.

Safety notes:

Intellectual property:

Corresponding part author/authors:

Source

Source:


Organism:


References

Literature references:

Database references:

  • [http://www.ncbi.nlm.nih.gov/nuccore/21435702 GenBank: source of the sequence]