Part:BBa_K823030

P_cotYZ: B. subtilis promoter regulating expression of spore crust proteins

P_cotYZ: Promoter of B. subtilis that is involved in spore crust formation. Hence it is activated in late sporulation. P_cotYZ regulates the crust protein cotZ and cotY.

Usage and Biology

Gene clusters of cotZ and cgeA

We used the promtoer P_cotYZ, P_cotV and P_cgeA for the regulation of spore crust proteins in B. subtilis.

Evaluation

To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

These are results of a plate reader assay to evaluate promoter activity. Promoters were cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter P_cotV (BBa_K823033) and P_cgeA.

The result of the measurement of the three promoters PcgeA, PcotV and PcotYZ. The left vertical axis displays the luminescence divided by the OD600 and the left one shows the OD600. On the horizontal axis the time is illustrated. The drawn through lines show the luminescence which correlates with the promoter activity and the dotted lines display the growth curve of each clone.

This part was used to display fusion proteins on B. subtilis spores with BBa_K823049.

See all the details about the LMU-Munich 2012 project here: http://2012.igem.org/Team:LMU-Munich/Spore_Coat_Proteins

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]