Part:BBa_K325909:Experience
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how you used this part and how it worked out.
Applications of BBa_K325909
[http://2012.igem.org/Team:Peking Peking 2012 iGEM team] |
We successfully constructed light communication system using this part-luxbrick, and it was the very first time that cells were able to response to very dim bio-luminesence emitted from luxbrick. Also we made supplementary characterization of it.
Figure 1. The treatment to each group (upper) and response of light sensing cell (lower) this photo shows the light emitting cell transformed with luxbrick in our light-communication system. Figure 2. TOP10 cells transformed with luxbrick from Cambridge 2010 iGEM team Also we made a short video to show the time course of our light communication system. See the video please click [http://www.youtube.com/watch?v=PCORfy8gJxM&feature=player_embedded here] See the detail of our light communicaiton system, please click [http://2012.igem.org/Team:Peking/Project/Communication here] |
User Reviews
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[http://2012.igem.org/Team:Fudan_Lux 2012iGEM team Fudan_Lux] |
Growth curve of K325909 In 2012 iGEM competition we measured growth curve of K325909 in a E.coli strain dH5α. The curve shows that induced by the arabinose, the bacteria growth Check the RAW data of measurement. |
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[http://2012.igem.org/Team:Peking/Project/Communication Peking 2012 iGEM team] |
We successfully constructed light communication system using this part-luxbrick, and we made supplementary characterization of it.
Figure 1. TOP10 cells harboring the luxbrick were cultured in LB medium and induced with L-arabinose at 10-3M. 10 hours after induction, the glowing cells were measured for spectrum using SHIMADZU RF5301PC Spectrofluorophotometer. (Peking iGEM 2012)
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UNIQ3d29d824c86964bc-partinfo-00000005-QINU UNIQ3d29d824c86964bc-partinfo-00000006-QINU