Device
aeBlue

Part:BBa_K864403

Designed by: Olle Nordesjö   Group: iGEM12_Uppsala_University   (2012-09-25)
Revision as of 23:49, 25 September 2012 by Rasinj (Talk | contribs) (added pictures)

J23110-B0034-aeBlue (chromoprotein) (E.coli optimized)

This part contains BBa_J23110, BBa_B0034 and BBa_K864401.

aeBlue is a blue chromoprotein extracted from the basal disk of a beadlet anemone Actinia equina. The protein has an absorption maximum at 597nm and a deep blue colour. The protein aeBlue is of the GFP family with significant sequence similarities.

Shkrob, M.A., Yanushevich, Y.G., Chudakov, D.M., Gurskaya, N.G., Labas, Y.A., Poponov, S.Y., Mudrik, N.N., Lukyanov, S., Lukyanov, K.A., 2005. Far-red fluorescent proteins evolved from a blue chromoprotein from Actinia equina. Biochem. J. 392, 649–654.


IMG 4371a.jpg AeBlue onplate.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//classic/reporter
//function/reporter
//function/reporter/color
//function/reporter/pigment
Parameters
abs597nm
device_typeReporter
familyGFP
functionColor
proteinaeCP597
uniprotQ2VFP3