Generator

Part:BBa_K792011

Designed by: Manuel Giménez   Group: iGEM12_Buenos_Aires   (2012-09-25)
Revision as of 15:04, 25 September 2012 by Manugimenez (Talk | contribs)

Yeast exportable His-rich peptide w/enhanced import (2)

This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.


The high level structure of the device is:

  • BamHI restriction site (introduced by device designers just to use this biobrick with an specific yeast vector - this is not essential for the device purpose)
  • Kozak consensus sequence for initiation of translation
  • Signal peptide that targets the product of the gene for secretion
  • Trojan peptide, to increase internalization in target cell (Polyarginine)
  • Payload: this is the exported Histidine rich domain of the protein (a custom designed peptide that we called PolyHb)


The device's sequence has inherited HindIII restriction site from the parts it's made of.


Important note: this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/yeast
Parameters
None