Generator
Part:BBa_K792011
Designed by: Manuel Giménez Group: iGEM12_Buenos_Aires (2012-09-25)
Yeast exportable His-rich peptide w/enhanced import (2)
This composite device produces and exports a Histidine rich peptide, that is import enhanced (thanks to a trojan peptide). This device is intended to be used with Yeast chassis, so it is composed by subparts designed also to be use in yeast.
The high level structure of the device is:
- BamHI restriction site (introduced to facilitate cloning into yeast expression vectors (pEG202, pCM180 series) - this is not essential for the device function)
- Kozak consensus sequence for initiation of translation
- Signal peptide that targets the product of the gene for secretion
- Trojan peptide, to increase internalization in target cell (Polyarginine)
- Payload: this is the exported Histidine rich domain of the protein (a custom designed peptide that we called PolyHb)
The device's sequence has inherited HindIII restriction site from the parts it's made of.
Important note: | this part has been submitted to the registry using a direct synthesis DNA sample, so it does not contain scars from BB assembly standard. |
See also: | Another His-rich peptide export and enhanced import composite device Part:BBa_K792009. |
Similar design Trp-rich peptide export and enhanced import composite device Part:BBa_K792012. |
This device contains BBa_K792004, a trojan peptide. Extreme your safety measures when dealing with strains containing this part: avoid direct contact with them or conditioned medium and discard them as pathogenic residues. |
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//chassis/eukaryote/yeast
None |