Reporter

Part:BBa_K864121

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala   (2012-09-25)
Revision as of 06:40, 25 September 2012 by Agynna (Talk | contribs)

pMB1 ori - constitutive RFP

This is a minimal high-copy replication origin coupled to a constitutive RFP casette. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation.


Usage and Biology

Useable as default insert of low and medium copy backbones, especially in lacI positive systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 1434
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None