Reporter

Part:BBa_K864121

Designed by: Arvid Hedén Gynnå   Group: iGEM12_Uppsala_University   (2012-09-25)
Appearance of pUCori-redq, in pSB4K15Iq and DH5α, on LA plate.

pMB1 ori - constitutive RFP

This is a minimal high-copy replication origin coupled to the constitutive RFP cassette BBa_K864502. Any vector containing K864121 will be propagated at high copy and express a strong red color after 12-18 hours of incubation. Also known as "pUCori-redq".


Usage and Biology

Usable as default insert of low and medium copy backbones, especially in lacI positive systems. This is the default insert of the pSB4x15Iq and pSB8x15 series of backbones.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 19
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1322
    Illegal AgeI site found at 1434
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None