Composite
psb1C3_int
Part:BBa_K743006:Design
Designed by: Group: iGEM12_UC_Chile (2012-09-20)
psb1C3_IntK recombination plasmid for Synechocystis PCC6803
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1774
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1774
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1774
Illegal XhoI site found at 2271 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1774
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1774
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
Source
RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance (BBa_P1003) and the double terminator (BBa_B0015) are from the registry of standard biological parts.