Composite
psb1C3_int
Part:BBa_K743006:Design
Designed by: Group: iGEM12_UC_Chile (2012-09-20)
psb1C3_IntK recombination plasmid for Synechocystis PCC6803
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1774
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1774
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1774
Illegal XhoI site found at 2271 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1774
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1774
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 511
Design Notes
Part BBa_K743001 has an EcoR1 restriction site, because of this, this plasmid can not accept biobricks by strandard assembly for further integration into Synechocystis. If adittion of any dna sequence is intended, Gibson assembly is strongly recomended.
You can check our experience with this plasmid and how we used it for Gibson assemblies at our [http://2012.igem.org/Team:UC_Chile wiki]
Source
RS1 and RS2 were obtained from Synechocystis genomic DNA, both the kanamycin resistance (BBa_P1003) and the double terminator (BBa_B0015) are from the registry of standard biological parts.