Device
FlipFlop

Part:BBa_K907002

Designed by: Dong-hui Choe, Soo-in Lee   Group: iGEM12_KAIST_Korea   (2012-09-20)
Revision as of 17:13, 20 September 2012 by Registry (Talk | contribs)

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Binary signal generator, RBS(reverse) - attB - Promoter - attP - RBS

Dual phase protein generator promoter reversed, RBS(reverse) - attB - Promoter(reverse) - attP - RBS

This part is composed of 3 elements. 1. RBS: BBa_B0034, upsteam one is reversed 2. Promoter: BBa_J23119, Bacterial constitutive promoter 3. att site: Recognition site for Mycobacteriophage Bxb1 integrase/excisionase We designed this part to generate two different proteins one at a time.

At the first state, this device promotes transcription and translation of downstream gene because its promoter orientation. When Mycobacteriophage Bxb1 integrase recognizes and inverts sequence flanking with attB and attP sequence, promoter orientation reversed. Then this device promotes transcription and translation of upstream gene(must be reversed at the construction step).

This device is tested by GFP and mRFP attached construct. See BBa_K090700N and BBa_K090700N

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 75
    Illegal NheI site found at 98
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 157
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 22
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 42
    Illegal BsaI.rc site found at 125


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/bidirectional
//promoter
//regulation/multiple
Parameters
None