Signalling
TD0.3

Part:BBa_K750011

Designed by: Sifan Wang,Ruosang Qiu,Yizhen Yan,Youbin Mo   Group: iGEM12_XMU-China   (2012-09-19)
Revision as of 11:50, 19 September 2012 by Registry (Talk | contribs)

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TIME DELAY0.3:LuxI(RBS0.3)->LuxR->LuxPR->GFP

This part contains part6 and part10. Arabinose will activate the promoter Pbad, which will cause production of protein LuxI and LuxR. The protein LuxI, LuxR and LuxPR make up the quorum sensing system, which leads to the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
    Illegal NheI site found at 1070
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 794
    Illegal BamHI site found at 65
    Illegal BamHI site found at 1010
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2025
    Illegal BsaI.rc site found at 2752


[edit]
Categories
Parameters
controlK206000
device_typesignaling
proteinsLuxI,LuxR,GFP
signalling_moleculeAHL