Part:BBa_K750011

TIME DELAY0.3:LuxI(RBS0.3)->LuxR->LuxPR->GFP

This part contains BBa_K750003 and BBa_K750007. Arabinose will activate the promoter pBAD, which will cause production of protein LuxI and LuxR. The protein LuxI will synthesize a small, diffusible signaling molecule acyl-homoserinelactone(AHL). The AHL accumulates as the cell density increases. At sufficiently high concentrations, it binds the LuxR, which induces the expression of our reporter GFP under the control of a promoter lux pR. Expression of LuxI will be affected when strength of RBS before luxI gene is changed. When expression of LuxI is weak, speed of AHL production will go down, that means it costs longer time for AHL-LuxR to arrive the activation thrshold of the promoter lux pR. In the time delay part of our project E.Lumoli, we designed 4 circuits with RBSes of different strength in the LuxI producer to construct a time delay device. Other time delay parts: TD1.0(BBa_K750009), TD0.6(BBa_K750010), TD0.01(BBa_K750012).
Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 125
  Illegal NheI site found at 1070
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 794
  Illegal BamHI site found at 65
  Illegal BamHI site found at 1010
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2025
  Illegal BsaI.rc site found at 2752