Part:BBa_K731701:Design

BBa_K731700 with T7terminator (BBa_K731721) This is the BBa_K731700 platform with the BBa_K731721 T7 terminator inserted in the BioBrick cloning region. This part was used in BBa_K731721 characterization.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal prefix found in sequence at 6850
  Illegal suffix found in sequence at 1
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 6850
  Illegal NheI site found at 6879
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 6856
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 6850
  Illegal BglII site found at 6011
  Illegal BamHI site found at 6832
  Illegal XhoI site found at 753
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found in sequence at 6850
  Illegal suffix found in sequence at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found in sequence at 6850
  Illegal XbaI site found at 6865
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 714
  Illegal NgoMIV site found at 1051
  Illegal NgoMIV site found at 4231
  Illegal NgoMIV site found at 4391
  Illegal NgoMIV site found at 5979
  Illegal AgeI site found at 6800
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 2228
  Illegal SapI.rc site found at 3310
  

Design Notes

The measurements with this plasmid were made using the E. coli strain BL21(DE3)pLysS. This is a lysogen strain that expresses T7 RNA polymerase. The expression in this strain give a very low basal expression.

Source

This is a composite part made of BBa_K731721 inserted in the BBa_K731700 backbone. The backbone is derived from the RL024A, a plasmid made by Roberta Lentini and kindly provided to the Trento iGEM team by the Mansy lab. The terminator has been extracted by PCR from pET21b vector.