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Part:BBa_K784018:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)
Revision as of 16:15, 16 September 2012 by Registry (Talk | contribs)

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Phage Lambda fragment 4 - tail fiber proteins


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal BglII site found at 2946
    Illegal BamHI site found at 2867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
    Illegal NgoMIV site found at 561
    Illegal AgeI site found at 144
    Illegal AgeI site found at 215
    Illegal AgeI site found at 2599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7755
    Illegal SapI.rc site found at 5290


Design Notes

Hila, update this.


Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1

The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp


References