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Part:BBa_K784018:Design

Designed by: Hila Korach Rechtman   Group: iGEM12_Technion   (2012-09-16)

Phage Lambda fragment 4 - tail fiber proteins


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal BglII site found at 2946
    Illegal BamHI site found at 2867
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1747
    Illegal EcoRI site found at 6625
    Illegal XbaI site found at 5029
    Illegal PstI site found at 354
    Illegal PstI site found at 802
    Illegal PstI site found at 2942
    Illegal PstI site found at 7449
    Illegal NgoMIV site found at 561
    Illegal AgeI site found at 144
    Illegal AgeI site found at 215
    Illegal AgeI site found at 2599
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7755
    Illegal SapI.rc site found at 5290


Design Notes

This bio-brick is one of eight correlated bio-bricks – each one contains a one fragment of the phage lambda CI875s7. The phages fragments created in order to allow manipulation in the phage genome by manipulation smaller fragments, and re-factor the complete 48kb genome after the manipulation.
The main goal with the genome deviation to eight fragments was to avoid putting regulatory elements with their regulated sequences. By doing so, we can prevent the expression of harmful and lytic associated proteins to the host bacteria. Moreover, due to the phage lambda genome organization we could divide the phages genome while each part contains several proteins which are in functional correlation with each other.

Source

http://www.ncbi.nlm.nih.gov/nuccore/NC_001416.1

The physical DNA was obtained from NEB - http://www.neb.com/nebecomm/products/productn3011.asp


References