Composite

Part:BBa_K784009

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-15)
Revision as of 14:09, 16 September 2012 by Sl.ilya (Talk | contribs)

Spacer2+RBS+mCherry

This part has been created by deleting the aptamer and the additional 5' UTR regions of the theophylline riboswitch using PCR. In addition, the deleted regions were replaced by a spacer region, which was added using the primers. The spacer region contains a PacI restriction site. As a result, only the RBS from the theophylline riboswitch remains.
Due to a mutation, this part differs from BBa_K784007.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None