Composite

Part:BBa_K784006

Designed by: Ilya Vainberg Slutskin   Group: iGEM12_Technion   (2012-09-13)
Revision as of 19:18, 13 September 2012 by Sl.ilya (Talk | contribs)

Theophylline riboswitch 12.1 + mCherry

This part is a direct fusion of the theophylline riboswitch (BBa_K784005) and the mCherry protein (BBa_J06504) which was amplified from BBa_J06702. The part was generated by [http://openwetware.org/wiki/Assembly_pcr Assembly pcr]. This part was to be used in order to characterize the effect of theophylline concentration on the level of translation of the mCherry protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 15
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//function/reporter
//function/reporter/fluorescence
Parameters
abs --
biology
emission610
emit610
excitation587
excite587
lum --
proteinmCherry2
tagNone