Regulatory
PcotYZ

Part:BBa_K823030:Experience

Designed by: Franziska Dürr   Group: iGEM12_LMU-Munich   (2012-09-06)
Revision as of 14:15, 7 September 2012 by Dexios (Talk | contribs) (→‎Applications of BBa_K823030)

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Applications of BBa_K823030

We used the promtoer PcotYZ for the regulation of spore crust proteins in B. subtilis. To test the activity we cloned this promoter into the lux-reporter vector pSBBS3C-luxABCDE (BBa_K823025) and measured the luminescence.

This part was used to display fusion proteins on B. subtilis spores.

These are results of a plate reader assay to evaluate promoter activity. PcotYZ was cloned into pSBBS3C-luxABCDE and luminescence was measured. This graph also shows activiy of the related promoter PcotV BBa_K823033

Activity PcotYZ PcotV.png

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UNIQ38642b2aa715b395-partinfo-00000000-QINU UNIQ38642b2aa715b395-partinfo-00000001-QINU