Regulatory

Part:BBa_M36233:Design

Designed by: Lawrence Xing   Group: Stanford BIOE44 - S11   (2011-12-10)
Revision as of 14:08, 4 July 2012 by Rshetty (Talk | contribs) (→‎Design Notes)

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Constitutive Promoter (E. coli)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Obtained from BIOFAB.org

First 34 bp is an exact match to the Bacteriophage T7 A1 promoter. See Accession X73047.1.

This sequence is missing the 5' insulator that the BioFAB used in their promoter vs 5' UTR designs. See pFAB3665.

Source

BIOFAB part id: apFAB85; BIOFAB construct id: pFAB329

References