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Part:BBa_K917001:Design

Designed by: Chris French   Group: iGEM12_Edinburgh   (2012-06-29)
Revision as of 14:45, 29 June 2012 by Registry (Talk | contribs)

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ars promoter plus cscA sucrose assimilation gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made by combining J33203 with K917000


Source

This part consists of the E. coli chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the cscA sucrose hydrolase gene from an E. coli O157 strain (K917000).

References