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Part:BBa_K917001:Design

Designed by: Chris French   Group: iGEM12_Edinburgh   (2012-06-29)

ars promoter plus cscA sucrose assimilation gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1042
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 255
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Made by combining J33203 with K917000


Source

This part consists of the E. coli chromosomal ars promoter and arsR repressor gene (J33201), which together comprise a promoter system induced by sodium arsenate, plus the lacZ' minigene (J33202) and the cscA sucrose hydrolase gene from an E. coli O157 strain (K917000).

References

Jahreis, K., Bentler, L., Bockmann, J., Hans, S., Meyer, A., Siepelmeyer, J., et al. (2002). Adaptation of sucrose metabolism in the Escherichia coli Wild-Type Strain EC31132. Journal of Bacteriology, 5307-5316.