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DNA
Part:BBa_M36301:Design
Designed by: Darren Jindal, Raman Nelakanti, Dennis Te Group: Stanford BIOE44 - S11 (2011-12-08)
Dual FseI restriction sites bounded by spacers
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal NgoMIV site found at 20 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This site was designed to guard against DNA methylation.
Source
FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence.
Nelson, J.M., Miceli, S.M., Lechevalier, M.P. and Robers, R.J. 1990. FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'. Nucleic Acids Res., 18, 2061-2064.