DNA

Part:BBa_M36301:Design

Designed by: Darren Jindal, Raman Nelakanti, Dennis Te   Group: Stanford BIOE44 - S11   (2011-12-08)

Dual FseI restriction sites bounded by spacers


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 20
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The spacers surrounding the restriction sites were designed to guard against DNA methylation.

Source

References

FseI, a new type II restriction endonuclease that recognizes the octanucleotide sequence. 

    Nelson, J.M., Miceli, S.M., Lechevalier, M.P. and Robers, R.J. 1990. FseI, a new type II restriction 
    endonuclease that recognizes the octanucleotide sequence 5' GGCCGGCC 3'. Nucleic Acids Res., 18, 2061-2064.