Measurement

Part:BBa_K669006

Designed by: Silvia J Cañas   Group: iGEM11_Colombia   (2011-10-28)
Revision as of 03:53, 29 October 2011 by Registry (Talk | contribs)

(diff) ↠Older revision | Latest revision (diff) | Newer revision → (diff)


Allows the measurement of the CBP promoter activity

Chitooligosaccharide-binding protein (CBP) is a cytoplasmatic protein that regulates the transcriptional activity of the ChiS sensor.

When chitin is present in the external media, CBP binds with GlcNAc oligomers (chitin degradation products) and liberates the sensor for the up-regulation of the chitinolitic degradation cascade in Vibrio fischeri ES114.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 119
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None