Measurement

Part:BBa_K669006

Designed by: Silvia J Cañas   Group: iGEM11_Colombia   (2011-10-28)

Allows the measurement of the CBP promoter activity

Chitooligosaccharide-binding protein (CBP) is a cytoplasmatic protein that regulates the transcriptional activity of the ChiS sensor.

When chitin is present in the external media, CBP binds with GlcNAc oligomers (chitin degradation products) and liberates the sensor for the up-regulation of the chitinolitic degradation cascade in Vibrio fischeri ES114.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 119
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 119
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 119
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 119
  • 1000
    COMPATIBLE WITH RFC[1000]

Circular specification. Registry curation 5-4-2014

This part was defined as a composite part Self - BBa_J06702. This definition is recursive and not valid. The designer should define a basic part with only the parts sequence and then define a separate composite part with the enclosing parts. The current part is just many copies of the mCherry part

We have redefined it as a basic part.


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Categories
Parameters
None