Regulatory
Pgad

Part:BBa_K559011

Designed by: Jacky, Fong Chuen, Loo   Group: iGEM11_Hong_Kong-CUHK   (2011-10-28)
Revision as of 21:24, 28 October 2011 by Jacky Loo (Talk | contribs) (→‎Usage and Biology)


Pgad - Intracellular Chloride-Sensing Cassette

Pgad is chloride-sensitive promoter which was first discovered in Lactococcus lactis1, which is a gram-positive bacterium which can live in acidic environment. In this operon, gadC is glutamate-gamma-aminobutyrate antiporter and gadB is glutamate decarboxylase. They are both involved in intracellular pH regulation and co-expressed in the same operon under the control of Pgad. The gene before Pgad, named gadR, which is constitutively expressed under the control of PgadR, is a positive regulator of Pgad coupled genes while intracellular chloride is level elevated.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

It has been used to fuse with GFP BBa_E0040 to quantitatively control GFP gene expression by providing different chloride concentration. The results can be seen in Part:BBa_K559011:Experience.


[edit]
Categories
//chassis/prokaryote/lactobacillus
//classic/regulatory/other
//classic/signalling/receiver
//collections/probiotics/control
//function/cellsignalling
Parameters
control
function
positive_regulators
receiver