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Part:BBa_K539392

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)
Revision as of 06:50, 8 October 2011 by Zcc (Talk | contribs)

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Vio operon ABDE(each preceded by their own ribosome binding site) with a terminator

Built in vioABCE (actually is vioABDE Part:BBa_K274004) and a terminator (Part:BBa_J61048).

We found out that there's a point mutation in the BBa_K274004 . This mutation occurs in the Xba I restriction site which takes a lot of time for us just to digest it in order to ligase this part with our interest part.

We also notice something that this part is actually vioABDE instead of vioABCE.

In previous time, we failed in every attempts to clone out vioC from this part. We then decided to do DNA sequencing to make sure every single sequence is correct. Afterward, we found the exact sequence of vioABDE instead of vioABCE.


The vioABDE catalyze L-Tryptophane to protoviolaceinic acid and produce a dark green thing. It can be added any part you want to improvise its fuction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal NgoMIV site found at 6040
    Illegal AgeI site found at 4010
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5239
    Illegal SapI.rc site found at 5314


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Parameters
n/aVio operon ABDE(each preceded by their own ribosome binding site) with a terminator