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Part:BBa_K274004

Designed by: Shuna Gould   Group: iGEM09_Cambridge   (2009-10-19)


Vio operon ABCE

Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction with BglII and BclI. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1029
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4289
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3814
    Illegal AgeI site found at 4010
  • 1000
    COMPATIBLE WITH RFC[1000]


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