Part:BBa_K118008:Experience

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UNIQ815145316abd644f-partinfo-00000000-QINU UNIQ815145316abd644f-partinfo-00000001-QINU

Characterisation by 2011 iGEM NCTU_FORMOSA

We found a point mutation of Part:BBa_K118008 (iGEM08_Edinburgh) at SpeI site and we corrected it successfully.(ACCTAGT->ACTAGT) This corrected part is numbered Part:BBa_K539119 Following is the original sequence of crtY Part:BBa_K118008. The SpeI mutate into ACCTAGT.
File:Mutate-1
Therefore, it need to delete the additional amino acid,C. The protocol we used is shown below.
Point Mutation
1.Design primers
2.Find the best PCR condition by gradient PCR
3.KOD PCR condition