Device

Part:BBa_K598017:Design

Designed by: Qingyang XIAO   Group: iGEM11_Peking_R   (2011-09-27)
Revision as of 06:19, 5 October 2011 by QingyangXiao (Talk | contribs) (→‎Source)

pBAD+Adenine Hammerhead Ribozyme+RBS+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 919


Design Notes

This part constructs by connecting adenine aptamer from add riboswitch with stem III of wildtype hammerhead ribozyme by a selected linker of twelve basepairs.

Source

The adenine aptamer was first found in V. vulnificus and the fast-cleaving hammerhead ribozyme is from Schistosoma mansonii

References