Signalling
Part:BBa_K574009
Designed by: Lei Wei Group: iGEM11_Tsinghua-A (2011-09-29)
3OC12HSL -> PoPS Receiver
This composite part can be considered as 3OC12HSL in -> PoPS out device, because 3OC12HSL binds LasR (BBa_C0079) which turns on pLas promoter(BBa_R0079). This device produces lasR constitutively, under the control of a constitutive regulated promoter (BBa_J23100). Device input is 3OC12HSL. Device output is PoPS from the Plas promoter(BBa_R0079).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 1048 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 386
Illegal AgeI site found at 583 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/signalling/receiver
//function/cellsignalling
//function/cellsignalling
device_type | Receiver |
signalling_molecule | 3OC12HSL |