Reporter
CFP opt

Part:BBa_K566003

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 18:02, 28 September 2011 by Janmatrey (Talk | contribs)

CFP optimized for E. coli

Cyan fluorescent protein optimized from part BBa_E0022 with preferential codon usage for improved expression in E. coli.

Usage and Biology

Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with exitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1, which means how strongly a chemical species absorbs light at a given wavelength. It has low absortion and low quantum yield (40%). The fluorescence quantum yield gives the efficiency of the fluorescence process. It is defined as the ratio of the number of photons emitted to the number of photons absorbed. 

   \Phi = \frac {\textrm{Number\ of\ photons\ emitted}} {\textrm{Number\ of\ photons\ absorbed}}


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None