Reporter
RFP opt

Part:BBa_K566013

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 08:08, 28 September 2011 by Janmatrey (Talk | contribs)

RFP optimized for E. coli (+LVA)

RFP optimized for E. coli. Includes LVA tag.

Preferential codons to improve their expression in E. coli.

Usage and Biology

Homotretameric protein from Discosoma sp. Thought to play a role in photoprotection of the coral's resident symbiont microalgae's photosystems from photoinhibition caused by high light levels found near the surface of coral reefs. In deeper water, the fluorescence may be to convert blue light into longer wavelengths more suitable for use in photosynthesis by the microalgal symbionts. It belongs to the GFP family. It possesses bright fluorescence with excitation/emission maxima at 558 and 583 nm, respectively. It has an extinction coefficient of 72,500 M-1 cm-1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 570
    Illegal AgeI site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None