Composite
Part:BBa_K539461:Design
Designed by: Shu-Han Chang Group: iGEM11_NCTU_Formosa (2011-09-27)
promoter (lacI regulated)+RNA thermometer+vioD with RNA thermometer+tetR+double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2119
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1194
Illegal SapI.rc site found at 1269
Design Notes
Built in Plac promoter(Part:BBa_R0010), followed by vioD(Part:BBa_K439413), 37’celcius regulator RBSs, tetR and terminator(Part:BBa_K332033).
Source
a