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Composite

Part:BBa_K539461:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated)+RNA thermometer+vioD with RNA thermometer+tetR+double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1194
    Illegal SapI.rc site found at 1269


Design Notes

Built in Plac promoter(Part:BBa_R0010), followed by vioD(Part:BBa_K539413), 37’celcius regulator RBSs, tetR and terminator(Part:BBa_K332033).

Source

The part which made of 37℃ induced RBS + tetR + double terminator(Part:BBa_K332033) belongs to 2010 iGEM NCTU_Formosa

References